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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance |
| Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immunologic testing methodologies - Immune system principles and components |
| Hematology | 20-25% | - White blood cell morphology and diseases - Hemostasis and coagulation testing - Routine and automated hematology procedures - Red blood cell physiology and disorders |
| Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Urine formation and composition - Physical, chemical, and microscopic analysis |
| Clinical Chemistry | 20-25% | - Toxicology and liver/kidney function tests - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases |
| Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Compatibility testing - Blood group systems and antigens - Antibody detection and identification - Transfusion reactions and adverse events |
| Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Specimen collection and culture techniques |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. In primary responses, the major class of antibody produced is IgM whereas in the secondary response, as mentioned in this question, it is IgG. IgG is present in the highest quantities compared to all other antibody classes and is the only antibody able to cross the placental barrier.
Which of the following antibody types is chiefly seen in the secondary immune response:
A) IgM
B) IgG
C) IgD
D) IgA
2. The purpose of protective isolation is to protect:
A) the patient from family conflicts
B) the phlebotomist after a needlestick
C) a compromised patient from infection
D) the phlebotomist from infection
3. Which of the following is a selective media recommended for the isolation of Clostridium difficile?
A) Thiosulphate citrate bile salts sucrose (TCBS) agar.
B) Cefoxitin-cycloserine-fructose agar (CCFA)
C) Buffered-charcoal yeast extract(BCYE) agar
D) Kanamycin Vancomycin laked blood (LKV) agar
4. Gamma globulin (anti-D) is given to Rh-negative mothers to prevent any D antibody production, which could cause harmful effects in future pregnancies with an Rh-positive fetus. The administered anti-D will bind fetal Rh-positive cells that may come from the fetus in vivo; therefore the mother will not produce anti-D herself, preventing sensitization.
A solution of gamma globulins containing anti-Rh (D) is given to an Rh (D) negative mother to:
A) Neutralize any natural maternal antibodies present
B) Prevent greater antibody response in a previously sensitized mother
C) Prevent fetal cells from initially sensitizing the mother
D) Neutralize any of the child's antibody that may have passed the placental barrier
5. Blood plasma will contain coagulation proteins, but not in a clotted tube where serum is present.
Blood serum contains each of the following substances except:
A) Electrolytes
B) Sugars
C) Water
D) Coagulation proteins
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: C | Question # 3 Answer: B | Question # 4 Answer: C | Question # 5 Answer: D |
